Name: Invoice Marano
Test Title: TLC Analysis of Analgesic Medications
Purpose: The purpose of this experiment is to discover the brand name of an not known commercial junk product by simply TLC evaluation. Unknown #1 solution was run against standard solutions of individual analgesics. The constituents of the unknown product had been identified by comparing characteristic traits, such as Rf, fluorescence and iodine staining, to people exhibited by the standards. The commercial product was after that determined using the reference table, Analgesics & Caffeine in Some Common Preparations. Reactions:
Method: Refer to Pavia, et. ing., Introduction to Organic and natural Laboratory Methods; 4th ed., Thomson Learning, pp. 82-86 Procedure Adjustments: In the Preliminary Preparations actions we utilized capillary tubes rather than planning capillary micropipets to spot the plates. All of us used 5cm x 20cm TLC china instead of 6cm x 10cm plates; this kind of required another type of slide structure. Also, simply 4 samples were noticed on each platter rather than 6. We analyzed 1 not known commercial item on menu two. Almost all standards were spotted two times and the guide samples and unknown had been spotted half a dozen times. In the Preparing the Development Chamber measures we applied large expanding chambers which usually we distributed to a partner instead of using the 16-oz. wide mouth jars. The Analysis of Commercial Analgesics or perhaps Unknowns steps were omitted since we included a mysterious on our second menu.
Observations and Data:
Total TLC development time was 40 minutes.
Desk 1: TLC Data intended for Standard Analgesics, Reference Mixture & Unidentified Components* Standard Substance| Distance Spot Moves (cm)| Length Solvent Front Travels (cm)| Fluorescence? | I2 Discoloration Appearance| Acetaminophen| 48| 150| No| Darker Yellow
Aspirin| 86| 150| No| Certainly not Significant
Caffeine| 14| 150| No| Not Significant
Ibuprofen| 92| 148| No| Not really Significant
Salicylamide| 81| 148| Yes|...